| Gene | Gpr44 |
| Chromosome | 19 |
| Deletion Start | 10,940,124 |
| Deletion End | 10,941,335 |
| Deletion Size | 1,212 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,940,439 | 10,940,459 | TCCTCGGTCTTCTTCCTCAAC |
| TUP | 10,940,461 | 10,940,483 | TGTTTGCCAGCGGCTTCCTGCTC |
| TUR | 10,940,489 | 10,940,507 | GCAGCGGTCCAGGCTAATG |
| TDF | 10,940,899 | 10,940,921 | CCTACCACATCTTCAGTCTGCTG |
| TDP | 10,940,923 | 10,940,944 | AGGCGCGTGCCCATTCTGTCAC |
| TDR | 10,940,978 | 10,940,997 | AGGCCAGGCTGGTGACAAAG |
PCR Primers
| Primer | Sequence |
| SU | CTCTAGCAAGCACTTACATTTC |
| SD | GGGAGTTATGAGATGCAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14756A-A9 | VGB6 | C57BL/6NTac | |
| 14756A-A11 | VGB6 | C57BL/6NTac | |
| 14756A-B7 | VGB6 | C57BL/6NTac | |
