| Gene | Sim1 |
| Chromosome | 10 |
| Deletion Start | 50,895,900 |
| Deletion End | 50,910,667 |
| Deletion Size | 14,768 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,895,980 | 50,895,998 | TTTACCCTCAGCCATCACC |
| TUP | 50,896,000 | 50,896,026 | CCCAACTGGACAAAGCATCCATCATCA |
| TUR | 50,896,054 | 50,896,075 | TTACCTTCTGGAAAGACCACTC |
| TDF | 50,909,910 | 50,909,932 | CCCACACTGCTCTCAATAGAACC |
| TDP | 50,909,934 | 50,909,958 | CGTGGGCTGAAGTCCATCAGATTGC |
| TDR | 50,909,982 | 50,910,002 | TCTCTGTACTGCTACCACCTC |
PCR Primers
| Primer | Sequence |
| SU | CCAGCTCAGAACCAGAGGAG |
| SD | ACCAGTAAATGGCTCCGTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14758A-E2 | VGB6 | C57BL/6NTac | |
| 14758A-B6 | VGB6 | C57BL/6NTac | |
