| Gene | Alg12 |
| Chromosome | 15 |
| Deletion Start | 88,816,228 |
| Deletion End | 88,805,757 |
| Deletion Size | 10,472 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,816,045 | 88,816,063 | AGGCACTGCACCGAGTGAG |
| TUP | 88,816,021 | 88,816,044 | CGGACGCTTGCTTCACTTGCCCAG |
| TUR | 88,815,972 | 88,815,993 | GAAACTCGTGGTGGTCGTACTG |
| TDF | 88,806,092 | 88,806,112 | TGGTGTGAGACGTAGGCAAAG |
| TDP | 88,806,061 | 88,806,088 | AATGCAGTGGTGACAGGTGACCTCTTTC |
| TDR | 88,806,027 | 88,806,048 | TGCTCCCACATCTTCACTCTTG |
PCR Primers
| Primer | Sequence |
| SU | AGGGGTCTTGTGCTTCCTCT |
| SD | CCTGGTTTATATCGTGGTCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14759A-G7 | VGB6 | C57BL/6NTac | |
| 14759A-C6 | VGB6 | C57BL/6NTac | |
| 14759A-F3 | VGB6 | C57BL/6NTac | |
| 14759B-G8 | VGB6 | C57BL/6NTac | |
| 14759B-A8 | VGB6 | C57BL/6NTac | |
| 14759B-F11 | VGB6 | C57BL/6NTac | |
| 14759B-E10 | VGB6 | C57BL/6NTac | |
| 14759B-A1 | VGB6 | C57BL/6NTac | |
| 14759B-G5 | VGB6 | C57BL/6NTac | |
