14763 9130008F23Rik Status: ES cell colonies screened / QC no positives
| Gene | 9130008F23Rik |
| Chromosome | 17 |
| Deletion Start | 40,880,533 |
| Deletion End | 40,879,902 |
| Deletion Size | 632 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,880,440 | 40,880,458 | GGGCCACCCTTGGATTCTG |
| TUP | 40,880,414 | 40,880,435 | CCCTAATCCTGCGCTGGCGGAG |
| TUR | 40,880,386 | 40,880,409 | CACTTGCTATTGATTGGGCACTTC |
| TDF | 40,880,028 | 40,880,047 | ACCGAGGAGCGTTCTGTGAC |
| TDP | 40,880,003 | 40,880,027 | CGCAGTCACCTTTCGGACACAGAGG |
| TDR | 40,879,932 | 40,879,954 | TCAGGTCCTTGGCGATAGGATAG |
PCR Primers
| Primer | Sequence |
| SU | TTGGGAAGGTCATTAGCACC |
| SD | ACACACCACACATCCCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 116 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
