| Gene | Nxnl2 |
| Chromosome | 13 |
| Deletion Start | 51,171,326 |
| Deletion End | 51,174,799 |
| Deletion Size | 3,474 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,172,228 | 51,172,249 | CCGCTTCTAGGACAGATGTCAG |
| TUP | 51,172,251 | 51,172,277 | TCCTGGGTATCCTTTAACTCTGGGTCC |
| TUR | 51,172,285 | 51,172,307 | GGTAGCATCGCAACTTGGATAAC |
| TDF | 51,174,637 | 51,174,658 | GAGGTACGAAATCACCGCCATC |
| TDP | 51,174,659 | 51,174,681 | CCCAAGCTGGTGGTCATCAAGCA |
| TDR | 51,174,700 | 51,174,720 | ATCTGCTTCCGCCCTTTGTTG |
PCR Primers
| Primer | Sequence |
| SU | TGGTGGGTAGAGAGGGTTTG |
| SD | TGCTCAAAGTGTGAGTGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14768A-A5 | VGB6 | C57BL/6NTac | |
| 14768A-C11 | VGB6 | C57BL/6NTac | |
| 14768A-E7 | VGB6 | C57BL/6NTac | |
| 14768A-F5 | VGB6 | C57BL/6NTac | |
| 14768A-H11 | VGB6 | C57BL/6NTac | |
