| Gene | Rdx |
| Chromosome | 9 |
| Deletion Start | 52,060,895 |
| Deletion End | 52,086,574 |
| Deletion Size | 25,680 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,061,688 | 52,061,710 | CCTGAGTTCAGTTCCCATCATCC |
| TUP | 52,061,712 | 52,061,737 | TGTTGGCCATGTTACAGCCTCCTGTA |
| TUR | 52,061,761 | 52,061,780 | CCAAGTGGGTCCCAGAAGAG |
| TDF | 52,086,412 | 52,086,432 | ACAAGACCCTTCGGCAGATTC |
| TDP | 52,086,434 | 52,086,459 | ACAAGGCAATACGAAGCAGCGCATCG |
| TDR | 52,086,464 | 52,086,486 | ACAGCTCTCACATGGCTTCAAAC |
PCR Primers
| Primer | Sequence |
| SU | TCCCTTAGACTTGAGGCTGC |
| SD | ACAACATGGAAAGGGCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14779A-F9 | VGB6 | C57BL/6NTac | |
| 14779A-D11 | VGB6 | C57BL/6NTac | |
| 14779A-E2 | VGB6 | C57BL/6NTac | |
