| Gene | Kbtbd5 |
| Chromosome | 9 |
| Deletion Start | 121,777,779 |
| Deletion End | 121,783,423 |
| Deletion Size | 5,645 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,778,649 | 121,778,669 | ACCAAGGCTGAGGACGATGAG |
| TUP | 121,778,671 | 121,778,694 | AACGAGTACTGCCCGGGATCCTCA |
| TUR | 121,778,699 | 121,778,716 | ATGCCGAAGCGCAGAGTG |
| TDF | 121,782,580 | 121,782,597 | TCAGCCTAGCTGGCACTC |
| TDP | 121,782,598 | 121,782,621 | TTTATGCCTTGGGTGGCTTTGCCA |
| TDR | 121,782,666 | 121,782,684 | GGGCTCACCTCCAGATGTC |
PCR Primers
| Primer | Sequence |
| SU | TCGAGAGACCTTCCAGTTC |
| SD | GCTAGATGCTCGGAAAGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14780A-A4 | VGB6 | C57BL/6NTac | |
| 14780A-A7 | VGB6 | C57BL/6NTac | |
| 14780A-B6 | VGB6 | C57BL/6NTac | |
| 14780A-D10 | VGB6 | C57BL/6NTac | |
| 14780A-G10 | VGB6 | C57BL/6NTac | |
| 14780A-G12 | VGB6 | C57BL/6NTac | |
