| Gene | Ggcx |
| Chromosome | 6 |
| Deletion Start | 72,414,410 |
| Deletion End | 72,430,156 |
| Deletion Size | 15,747 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,415,144 | 72,415,166 | GGTTTGTTCAACCTGCTTTCTTC |
| TUP | 72,415,168 | 72,415,189 | ACCAGTAGGGATCACCTGCCCA |
| TUR | 72,415,223 | 72,415,243 | TGGCCCGTAAGCATTTATGTG |
| TDF | 72,429,509 | 72,429,528 | CCAACACCTCTGGTCCAAAC |
| TDP | 72,429,530 | 72,429,555 | TTTCTCAGACGACAGAGGAAGCTCCA |
| TDR | 72,429,582 | 72,429,603 | CGGAGAAATCGCTCATGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | AAGCAGGCGTCGAGATTG |
| SD | AGAACTTATAAGGGGAAAAAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14784A-B11 | VGB6 | C57BL/6NTac | |
| 14784A-D9 | VGB6 | C57BL/6NTac | |
| 14784A-D4 | VGB6 | C57BL/6NTac | |
| 14784A-F12 | VGB6 | C57BL/6NTac | |
