14790   Hoxd13     Status: ES cell colonies screened / QC positives

GeneHoxd13
Chromosome2
Deletion Start74,668,313
Deletion End74,670,150
Deletion Size1,838
Genome Build38
MGI IDMGI:96205
NCBI Gene ID15433
BAC ID130J7
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,668,68474,668,702CCACTTCGGCAACGGTTAC
TUP74,668,70374,668,723TACAGCTGCCGCATGTCGCAC
TUR74,668,73974,668,759GGACGACTTGAGAGCGTTCTG
TDF74,669,86974,669,893GCTGTTGTTTATGCTAGGAGATGTG
TDP74,669,89574,669,920CTTTAAACCAGCCGGACATGTGCGTC
TDR74,669,96874,669,992GGCATACTCATTCTCCAGTTCTTTG

PCR Primers

PrimerSequence
SUCTCCCAACCTGAACTTCGTT
SDCATACTGGCCTGGAGGGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR76 bp++-
TDFTDR124 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
14790A-G3VGB6C57BL/6NTac
14790A-H8VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image