| Gene | Prom1 |
| Chromosome | 5 |
| Deletion Start | 44,094,611 |
| Deletion End | 44,094,400 |
| Deletion Size | 212 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,094,584 | 44,094,602 | TTCAGTGCCCTGCTGTTAC |
| TUP | 44,094,547 | 44,094,575 | TGTGGAAAGATCTCTTCAGAAGGTCAGCC |
| TUR | 44,094,523 | 44,094,544 | GCCCCAGGAGTGTTATGGAATG |
| TDF | 44,094,477 | 44,094,501 | CACCAAATATGAGACCCAAGATACC |
| TDP | 44,094,452 | 44,094,475 | TCAATGCTGGGATTGTTGGCCCTC |
| TDR | 44,094,414 | 44,094,435 | CTGGACCACGTTGAGGAAGATG |
PCR Primers
| Primer | Sequence |
| SU | AGGAGCCTCTTCCTAGTCCC |
| SD | AAGCAATGTGTTGCTCTAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14792A-F1 | VGB6 | C57BL/6NTac | |
| 14792A-E3 | VGB6 | C57BL/6NTac | |
