| Gene | Bcl2l10 |
| Chromosome | 9 |
| Deletion Start | 75,347,906 |
| Deletion End | 75,351,210 |
| Deletion Size | 3,305 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,348,400 | 75,348,420 | GGCACAGGAGGATGTGTTGAG |
| TUP | 75,348,421 | 75,348,447 | TGCCTTTACCGCAGGGTCTTATGTAGC |
| TUR | 75,348,461 | 75,348,485 | GGGTAGTATCTGGTGAGGTTTGAGG |
| TDF | 75,350,837 | 75,350,858 | TGGGCAGTTGTGAGACACTATG |
| TDP | 75,350,863 | 75,350,886 | TGCTGGGCACGGAACCCAAATCTT |
| TDR | 75,350,905 | 75,350,926 | GGGATGGCTCCGAAGTTAAGAG |
PCR Primers
| Primer | Sequence |
| SU | CCTGGGACAACCACTTGC |
| SD | AGGGATTCCTTCCAGTTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14793A-C1 | VGB6 | C57BL/6NTac | |
| 14793A-D5 | VGB6 | C57BL/6NTac | |
| 14793A-D8 | VGB6 | C57BL/6NTac | |
| 14793A-D9 | VGB6 | C57BL/6NTac | |
| 14793A-F9 | VGB6 | C57BL/6NTac | |
| 14793A-H4 | VGB6 | C57BL/6NTac | |
