| Gene | Inhba |
| Chromosome | 13 |
| Deletion Start | 16,017,109 |
| Deletion End | 16,027,126 |
| Deletion Size | 10,018 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 16,017,596 | 16,017,618 | GTGGAGATAGAGGACGACATTGG |
| TUP | 16,017,620 | 16,017,648 | AGGAGGGCCGAAATGAATGAACTCATGGA |
| TUR | 16,017,670 | 16,017,689 | ACCCACCTGACTCGGCAAAG |
| TDF | 16,026,495 | 16,026,514 | GGAAGAGTACCTGGCACATC |
| TDP | 16,026,515 | 16,026,537 | TTTCCAGTGTCCAGCAGCATCCA |
| TDR | 16,026,582 | 16,026,600 | TCCTGGCACTGCTCACAAG |
PCR Primers
| Primer | Sequence |
| SU | TACTCGGAGACAGTCAGAAC |
| SD | CAAGAGGCAGGAGAGAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14798A-D9 | VGB6 | C57BL/6NTac | |
