| Gene | Tcirg1 |
| Chromosome | 19 |
| Deletion Start | 3,904,698 |
| Deletion End | 3,896,113 |
| Deletion Size | 8,586 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,904,284 | 3,904,304 | CCATCACAGCTCAACGAATCC |
| TUP | 3,904,262 | 3,904,282 | TGAGCGCCTTCCAGAGACGCT |
| TUR | 3,904,208 | 3,904,232 | CCAACTTACTAAACGTCTTCTCCAG |
| TDF | 3,896,683 | 3,896,702 | TCATGCACCAAGCAATCCAC |
| TDP | 3,896,658 | 3,896,682 | ACCATTGAGTTCTGCCTGGGCTGCA |
| TDR | 3,896,623 | 3,896,641 | GGCCCAGAGACGCAAGTAG |
PCR Primers
| Primer | Sequence |
| SU | AGACAGCCTACGGTGGTAG |
| SD | AATACAGGGCCAGTTTGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14800A-A2 | VGB6 | C57BL/6NTac | |
| 14800A-H7 | VGB6 | C57BL/6NTac | |
| 14800A-B12 | VGB6 | C57BL/6NTac | |
| 14800A-C5 | VGB6 | C57BL/6NTac | |
| 14800A-C9 | VGB6 | C57BL/6NTac | |
| 14800A-F1 | VGB6 | C57BL/6NTac | |
