| Gene | Atp6v0d2 |
| Chromosome | 4 |
| Deletion Start | 19,922,494 |
| Deletion End | 19,878,196 |
| Deletion Size | 44,299 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,922,425 | 19,922,445 | GCCTGGTTCGAGGATGCAAAG |
| TUP | 19,922,392 | 19,922,420 | CCTCCTAACTCAGCAGGACTATGTCAACC |
| TUR | 19,922,347 | 19,922,367 | GGGATGAGGTGAGTGGCTTAC |
| TDF | 19,878,551 | 19,878,572 | ACATTTGTCCATGTGACCCTTC |
| TDP | 19,878,519 | 19,878,546 | AGATGTGCTGAGTTGCCTCTGATCCCAC |
| TDR | 19,878,467 | 19,878,488 | CTGCTACAGTGGTGCCTATTTG |
PCR Primers
| Primer | Sequence |
| SU | CAACAACCTGGTGTCACTGG |
| SD | AATGCATGGAAGGACCAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14801B-G9 | VGB6 | C57BL/6NTac | |
| 14801C-D2 | VGB6 | C57BL/6NTac | |
| 14801B-C7 | VGB6 | C57BL/6NTac | |
