| Gene | Dcst1 |
| Chromosome | 3 |
| Deletion Start | 89,365,151 |
| Deletion End | 89,350,236 |
| Deletion Size | 14,916 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,364,300 | 89,364,319 | GCTGCTCCTCTGGAGTCTTC |
| TUP | 89,364,273 | 89,364,296 | TTTCCTGGATCCGGTTCCTGTGGC |
| TUR | 89,364,181 | 89,364,202 | TGCCCTTCAGCTTCCATACTTG |
| TDF | 89,350,802 | 89,350,819 | TTGTGCCCTCCTCCCTTC |
| TDP | 89,350,767 | 89,350,794 | AAGTACCAAATTAAGCCGCTCCACAGGT |
| TDR | 89,350,720 | 89,350,738 | GGCGTGCCCAAACTTACTC |
PCR Primers
| Primer | Sequence |
| SU | AAGCTCTAGGAGCCCAAAGG |
| SD | AGTCCCAGAGCCCAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 139 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14803A-D8 | VGB6 | C57BL/6NTac | |
| 14803A-E7 | VGB6 | C57BL/6NTac | |
| 14803A-B9 | VGB6 | C57BL/6NTac | |
| 14803A-G4 | VGB6 | C57BL/6NTac | |
