| Gene | Ecm2 |
| Chromosome | 13 |
| Deletion Start | 49,514,826 |
| Deletion End | 49,531,611 |
| Deletion Size | 16,786 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,514,911 | 49,514,935 | TGCATCAGAGAAGACTCAGGAAAAG |
| TUP | 49,514,940 | 49,514,964 | TCATTCCACCTCAGAGCCAACAGGC |
| TUR | 49,514,979 | 49,514,998 | TCTGGAGCAGCAGTGGTTTG |
| TDF | 49,530,901 | 49,530,925 | CGGTTTCAGATCAACCTTTAGGAAG |
| TDP | 49,530,929 | 49,530,949 | CCCTTCACCTTGCAGGCTGCA |
| TDR | 49,530,990 | 49,531,013 | CAGTAGAGACAGTAGAGGCAAGTG |
PCR Primers
| Primer | Sequence |
| SU | GTCTAGAGAGACAGATATTCC |
| SD | ATCTTGCTGCAGAATGAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14804A-C3 | VGB6 | C57BL/6NTac | |
| 14804A-E9 | VGB6 | C57BL/6NTac | |
