| Gene | Ecm1 |
| Chromosome | 3 |
| Deletion Start | 95,739,439 |
| Deletion End | 95,734,137 |
| Deletion Size | 5,303 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,738,841 | 95,738,860 | TCGGTTCAGCTCCAGATGTG |
| TUP | 95,738,817 | 95,738,840 | AAGGTGCTTTGCCAGCCTGGTGAC |
| TUR | 95,738,793 | 95,738,814 | GTGGGTTACTGGGACGGAATAC |
| TDF | 95,734,654 | 95,734,674 | CTTCCCGAGAACCTCTTGTCC |
| TDP | 95,734,633 | 95,734,653 | CTGCCCTGGGCCTCAAGCTTC |
| TDR | 95,734,565 | 95,734,584 | TGCCAGCTTCCTTTCCACAG |
PCR Primers
| Primer | Sequence |
| SU | ATGCAGATGGGAAACTGAGG |
| SD | TGCATATCTCCATGCACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14805A-A6 | VGB6 | C57BL/6NTac | |
| 14805A-B3 | VGB6 | C57BL/6NTac | |
| 14805A-H11 | VGB6 | C57BL/6NTac | |
| 14805A-C10 | VGB6 | C57BL/6NTac | |
| 14805A-G1 | VGB6 | C57BL/6NTac | |
| 14805A-H2 | VGB6 | C57BL/6NTac | |
