| Gene | Xcl1 |
| Chromosome | 1 |
| Deletion Start | 164,935,487 |
| Deletion End | 164,931,703 |
| Deletion Size | 3,785 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,935,466 | 164,935,487 | AGACTTCTCCTCCTGACTTTCC |
| TUP | 164,935,445 | 164,935,465 | TGGGAGTCTGCTGCCTCACCC |
| TUR | 164,935,385 | 164,935,407 | ATGAGCCCACCTCTTCTTTATCC |
| TDF | 164,931,798 | 164,931,817 | TCCACCAGCACAGCGATAAC |
| TDP | 164,931,772 | 164,931,797 | CCTGACTGGGTAACAGCCTCCAGGAC |
| TDR | 164,931,722 | 164,931,742 | GCAATGGGTTTGGGAACTGAG |
PCR Primers
| Primer | Sequence |
| SU | TTCAGGATGGAAGATCCGAC |
| SD | GGAAACTGAAATAGATCGAGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14808A-A7 | VGB6 | C57BL/6NTac | |
| 14808A-E11 | VGB6 | C57BL/6NTac | |
| 14808A-H9 | VGB6 | C57BL/6NTac | |
| 14808A-F4 | VGB6 | C57BL/6NTac | |
| 14808A-H5 | VGB6 | C57BL/6NTac | |
| 14808A-G8 | VGB6 | C57BL/6NTac | |
