14810   Wnt10a     Status: Germline transmission achieved

GeneWnt10a
Chromosome1
Deletion Start74,792,174
Deletion End74,803,688
Deletion Size11,515
Genome Build38
MGI IDMGI:108071
NCBI Gene ID22409
BAC ID49P9
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,793,05974,793,082GTATGCCTGAAGAAATGCCAGTTG
TUP74,793,08474,793,109TGAAACATAGCTTTGGAGCAGGCTCC
TUR74,793,11874,793,139CCCAGACACAGATGCCTCAAAG
TDF74,803,60174,803,619GGGTCAGCGTCTGCAAGTG
TDP74,803,62674,803,649CCCAAGCTCCTCTGGGTCTCAAGA
TDR74,803,65974,803,678CAGAAGCCAGGCACCAAGAG

PCR Primers

PrimerSequence
SUACCTCCTTACCCTCTAGAG
SDTGCATTGCCTATCACTCCTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR81 bp++-
TDFTDR78 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
14810A-A3VGB6C57BL/6NTac
14810A-A9VGB6C57BL/6NTac
14810A-B8VGB6C57BL/6NTac
14810A-F11VGB6C57BL/6NTac
14810A-E4VGB6C57BL/6NTac
14810A-D3VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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