| Gene | Ccl27a |
| Chromosome | 4 |
| Deletion Start | 41,773,779 |
| Deletion End | 41,769,413 |
| Deletion Size | 4,367 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GACTTTAGCGCCGCAGC |
| TUP | 41,773,128 | 41,773,152 | CTTTGGTGCTGGGAATCCCACTCCA |
| TUR | 41,773,095 | 41,773,115 | AGGGATGCCCTGTGGGTTAAC |
| TDF | 41,769,564 | 41,769,584 | CAAGGGAAAAGGCTCCAAGGG |
| TDP | 41,769,540 | 41,769,563 | ACTGTACCCAGTTTAAATCTGGTA |
| TDR | 41,769,493 | 41,769,518 | CTTTATTAGTTTTGCTGTTGGGGGTT |
PCR Primers
| Primer | Sequence |
| SU | CCTCCCTCTTTGGTGACTCC |
| SD | GAATTCCAAACCTGCAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14813A-B4 | VGB6 | C57BL/6NTac | |
| 14813A-E3 | VGB6 | C57BL/6NTac | |
| 14813A-E12 | VGB6 | C57BL/6NTac | |
| 14813A-F9 | VGB6 | C57BL/6NTac | |
