| Gene | Spink4 |
| Chromosome | 4 |
| Deletion Start | 40,920,098 |
| Deletion End | 40,931,391 |
| Deletion Size | 11,294 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,920,384 | 40,920,408 | AGGATGCCATTTCAGTTTGAAGATG |
| TUP | 40,920,409 | 40,920,436 | TCTTGAGCAATTATGGACTGGAGGCTGA |
| TUR | 40,920,440 | 40,920,461 | GGGCCTGACTGTTCCTTCTATC |
| TDF | 40,930,990 | 40,931,011 | GGTCCAAGAACAGACAGGACAG |
| TDP | 40,931,015 | 40,931,037 | TTTGGGCAGCCATCAGCAAGCAG |
| TDR | 40,931,055 | 40,931,077 | CACTCTGAGAACTTGCAGGTTTC |
PCR Primers
| Primer | Sequence |
| SU | CTTCCTCTCCCTCCCACTTC |
| SD | TACTGCCTGAATGCCATCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14814A-G2 | VGB6 | C57BL/6NTac | |
| 14814A-A11 | VGB6 | C57BL/6NTac | |
| 14814A-D10 | VGB6 | C57BL/6NTac | |
| 14814A-E1 | VGB6 | C57BL/6NTac | |
| 14814A-F3 | VGB6 | C57BL/6NTac | |
| 14814A-A4 | VGB6 | C57BL/6NTac | |
