| Gene | Lamc3 |
| Chromosome | 2 |
| Deletion Start | 31,887,347 |
| Deletion End | 31,916,078 |
| Deletion Size | 28,732 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,888,138 | 31,888,157 | GCAGGACAGCCAGTTTCTTC |
| TUP | 31,888,161 | 31,888,182 | CCACTGGATAGCCAGCCTGGGC |
| TUR | 31,888,212 | 31,888,232 | CTCAGTCTGGGTGGAGCAATC |
| TDF | 31,915,303 | 31,915,322 | TCCCGTCCCTCATCTTCCTG |
| TDP | 31,915,323 | 31,915,344 | TGAGCACTGCTCACCCTGCTGG |
| TDR | 31,915,346 | 31,915,365 | GAGGTGCAATGCCACTGTTG |
PCR Primers
| Primer | Sequence |
| SU | ACACTGGACAGGTACGGAGG |
| SD | TGGGAAGCAAGGCTCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14815A-H4 | VGB6 | C57BL/6NTac | |
| 14815A-F8 | VGB6 | C57BL/6NTac | |
| 14815A-F5 | VGB6 | C57BL/6NTac | |
| 14815A-F1 | VGB6 | C57BL/6NTac | |
| 14815A-A9 | VGB6 | C57BL/6NTac | |
| 14815A-C9 | VGB6 | C57BL/6NTac | |
