| Gene | Klk7 |
| Chromosome | 7 |
| Deletion Start | 43,811,964 |
| Deletion End | 43,815,530 |
| Deletion Size | 3,567 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,812,831 | 43,812,849 | GGACCAGAGTGCCCAGAAG |
| TUP | 43,812,851 | 43,812,877 | TCAAGGCTACAAAGTCATTCCGGCACC |
| TUR | 43,812,916 | 43,812,936 | TGGCTCATCCAACCTCACAAG |
| TDF | 43,814,860 | 43,814,882 | GATGCTATGCTGTATGCTGAAGG |
| TDP | 43,814,889 | 43,814,915 | TGGTGGAAGACACATTTCTCTTGCTGA |
| TDR | 43,814,931 | 43,814,950 | TGACTGTAGCCAAGGGAGAG |
PCR Primers
| Primer | Sequence |
| SU | CTAGACAGGAGGCCATGAGG |
| SD | ATGAGAGCAGTCCAACTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14816A-B7 | VGB6 | C57BL/6NTac | |
| 14816A-B9 | VGB6 | C57BL/6NTac | |
| 14816A-C5 | VGB6 | C57BL/6NTac | |
| 14816A-E5 | VGB6 | C57BL/6NTac | |
| 14816A-H10 | VGB6 | C57BL/6NTac | |
