Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

14821 Pla2g12b Status: ES cell colonies screened / QC positives

Gene	Pla2g12b
Chromosome	10
Deletion Start	59,403,774
Deletion End	59,421,639
Deletion Size	17,866
Genome Build	38

MGI ID	MGI:1917086
NCBI Gene ID	69836
BAC ID	268F4
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	59,404,551	59,404,572	CCAGAGACACAACTGGGAACTG
TUP	59,404,574	59,404,597	AGTGAGCTCCCATGCCTACCTTGC
TUR	59,404,600	59,404,620	CCCTAGCTGGCTTTCCAACTC
TDF	59,421,458	59,421,477	TCAAGCAGCCTGTGATTCTC
TDP	59,421,478	59,421,502	TGGCTGATACCGTGTTCAACACCGT
TDR	59,421,524	59,421,546	CTGCCCGCTGACTGTTCATAAAG

PCR Primers

Primer	Sequence
SU	GTGGCTGAGATCTTGGGAAG
SD	TTACTTACCGGCCTGCATTC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	70 bp	+	+	-
TDF	TDR	89 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
14821A-A1	VGB6	C57BL/6NTac
14821A-B12	VGB6	C57BL/6NTac
14821A-G4	VGB6	C57BL/6NTac
14821A-C9	VGB6	C57BL/6NTac