| Gene | Fuca2 |
| Chromosome | 10 |
| Deletion Start | 13,501,171 |
| Deletion End | 13,514,922 |
| Deletion Size | 13,752 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,501,307 | 13,501,329 | CCAAGTTCGGCATCTTTATCCAC |
| TUP | 13,501,335 | 13,501,358 | AGTGTTCTCCGTGCCCAGCTTTGG |
| TUR | 13,501,374 | 13,501,395 | TTGGGCAAAGGTATGCACTTAC |
| TDF | 13,514,680 | 13,514,704 | CATTGTTGTCCCTGTTTCCACTTAC |
| TDP | 13,514,708 | 13,514,729 | TAGAACTGCTGGGCCATTGGCA |
| TDR | 13,514,742 | 13,514,762 | CCACTGGGCTGTGAAGATGTC |
PCR Primers
| Primer | Sequence |
| SU | ATCTGACTGGGAAACAGAGC |
| SD | TAGGAGTTTCTAGCTTCTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14823A-F10 | VGB6 | C57BL/6NTac | |
| 14823A-F11 | VGB6 | C57BL/6NTac | |
| 14823A-G7 | VGB6 | C57BL/6NTac | |
| 14823A-G5 | VGB6 | C57BL/6NTac | |
| 14823A-D3 | VGB6 | C57BL/6NTac | |
