| Gene | Pgc |
| Chromosome | 17 |
| Deletion Start | 47,726,909 |
| Deletion End | 47,734,484 |
| Deletion Size | 7,576 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,727,018 | 47,727,038 | TCTGGTGCCTATGAAGGTGTC |
| TUP | 47,727,044 | 47,727,069 | CCCTGGGACAGGCATTACAACAGGTT |
| TUR | 47,727,081 | 47,727,099 | GAAGTGCAGGGTTCGAGTC |
| TDF | 47,733,831 | 47,733,853 | GTCACCCTCTTCCTACATCATCC |
| TDP | 47,733,857 | 47,733,882 | TAAGTTCTCACTCCTCTGGGCAGAGC |
| TDR | 47,733,888 | 47,733,907 | TCCAGACCCACACCAAATCC |
PCR Primers
| Primer | Sequence |
| SU | TCTTTGCCACTTCACTGTGC |
| SD | ACTTGGCATCTGAGTGACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14824A-F6 | VGB6 | C57BL/6NTac | |
| 14824A-C4 | VGB6 | C57BL/6NTac | |
| 14824A-E12 | VGB6 | C57BL/6NTac | |
| 14824A-D8 | VGB6 | C57BL/6NTac | |
