| Gene | Cpa4 |
| Chromosome | 6 |
| Deletion Start | 30,568,397 |
| Deletion End | 30,591,019 |
| Deletion Size | 22,623 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,569,216 | 30,569,238 | GGAAGCTGCCCAAAGCTACTTAG |
| TUP | 30,569,244 | 30,569,267 | CAGCACGGAAGCTGGAATGCTGAC |
| TUR | 30,569,289 | 30,569,313 | TCCATTTGATTGGCTTGTGTTACAG |
| TDF | 30,590,363 | 30,590,383 | GCCTTGTTAGGCAGCTTGTTC |
| TDP | 30,590,390 | 30,590,414 | TACCCTGACTGCCTCCTGAACGCTG |
| TDR | 30,590,415 | 30,590,434 | TGGTGGCCCACTTGTGATTG |
PCR Primers
| Primer | Sequence |
| SU | CTGAGATGCCAAGTGATGAAC |
| SD | GCCCTACCCAAGGCTACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14826A-H6 | VGB6 | C57BL/6NTac | |
| 14826A-G11 | VGB6 | C57BL/6NTac | |
| 14826A-B1 | VGB6 | C57BL/6NTac | |
| 14826A-D1 | VGB6 | C57BL/6NTac | |
