| Gene | Chit1 |
| Chromosome | 1 |
| Deletion Start | 134,138,751 |
| Deletion End | 134,151,399 |
| Deletion Size | 12,649 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,138,805 | 134,138,823 | GCCTCAGGAGCCATTGGTG |
| TUP | 134,138,826 | 134,138,848 | CGAGCCTGTGGTTCCCTCCTGCT |
| TUR | 134,138,900 | 134,138,920 | AACAGCCAAGCCAGGAGTCAG |
| TDF | 134,151,075 | 134,151,096 | CATTGCTTGAATCGTCCTTGTC |
| TDP | 134,151,099 | 134,151,127 | TCTAGATCTTCCATCCGAGACTCCAAGGA |
| TDR | 134,151,164 | 134,151,182 | GGTCCCTGCTCTGGCATAG |
PCR Primers
| Primer | Sequence |
| SU | CGGGAGTTGCTTGTCTTCTC |
| SD | CATCGGAAATGCAACTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 116 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14827A-C5 | VGB6 | C57BL/6NTac | |
| 14827A-C12 | VGB6 | C57BL/6NTac | |
| 14827A-A4 | VGB6 | C57BL/6NTac | |
| 14827A-F11 | VGB6 | C57BL/6NTac | |
| 14827A-E9 | VGB6 | C57BL/6NTac | |
