| Gene | Smpdl3b |
| Chromosome | 4 |
| Deletion Start | 132,757,042 |
| Deletion End | 132,733,376 |
| Deletion Size | 23,667 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 132,756,583 | 132,756,605 | GCTCTGAGGTACAGTGGTTGTTC |
| TUP | 132,756,557 | 132,756,579 | TGAGCGCGTTCCATCTCTGGGAC |
| TUR | 132,756,490 | 132,756,510 | TCCTTCCACCGTATGGGTTTG |
| TDF | 132,733,778 | 132,733,799 | CAACTGCTCGTTGCTGACATAC |
| TDP | 132,733,753 | 132,733,777 | CCTGCGCCTCTAATCCAGGACTTGG |
| TDR | 132,733,724 | 132,733,742 | CGCCTGCCTCAGGTTCAAG |
PCR Primers
| Primer | Sequence |
| SU | AACTCCAACACAGCACCTTG |
| SD | ACCACGTACGTTCCCAGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 116 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14831B-H10 | VGB6 | C57BL/6NTac | |
| 14831B-H12 | VGB6 | C57BL/6NTac | |
