14837 A2m Status: ES cell colonies screened / QC no positives
| Gene | A2m |
| Chromosome | 6 |
| Deletion Start | 121,636,292 |
| Deletion End | 121,662,438 |
| Deletion Size | 26,147 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,636,375 | 121,636,394 | TGCCTCAGCACCACAGAAAC |
| TUP | 121,636,397 | 121,636,419 | TGAGTCCACCGAACGCCAGAAAC |
| TUR | 121,636,492 | 121,636,513 | GTACAAATCTCGGCGCTCTAAG |
| TDF | 121,661,450 | 121,661,470 | GCAGAGGCACTGGACTCTAAG |
| TDP | 121,661,479 | 121,661,503 | TAGGAATGAGGTGCCAGTGGTTCCT |
| TDR | 121,661,527 | 121,661,547 | TCAACCAGCAGGGACTTGATG |
PCR Primers
| Primer | Sequence |
| SU | CAACTCTCCAGACAGGAGCC |
| SD | AGTTAGAGCCTTGTCTTTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 139 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
