| Gene | Kng2 |
| Chromosome | 16 |
| Deletion Start | 23,028,906 |
| Deletion End | 22,997,028 |
| Deletion Size | 31,879 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,028,765 | 23,028,791 | CTCTGAAGAAGCTTAATGCTAGGTTAC |
| TUP | 23,028,724 | 23,028,746 | TGTTGTACCGAGTGACCAAGCGC |
| TUR | 23,028,698 | 23,028,723 | GGAAAGCTGTTACTCACCATTTTAGC |
| TDF | 22,997,739 | 22,997,768 | GGATTTTCTGATTCAGTATTTAGCAGAGAG |
| TDP | 22,997,686 | 22,997,708 | AAGTGTTTCTTCTCATACCTCAG |
| TDR | 22,997,648 | 22,997,671 | CCTACCAAGTAACTCAAGGGCTTA |
PCR Primers
| Primer | Sequence |
| SU | GAGAAATGGGCTTTGCACTC |
| SD | CACACAGTAGTGCTAAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 121 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14840A-B9 | VGB6 | C57BL/6NTac | |
| 14840A-A12 | VGB6 | C57BL/6NTac | |
| 14840A-G5 | VGB6 | C57BL/6NTac | |
| 14840A-H6 | VGB6 | C57BL/6NTac | |
