| Gene | Pla2g2c |
| Chromosome | 4 |
| Deletion Start | 138,731,588 |
| Deletion End | 138,743,781 |
| Deletion Size | 12,194 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,731,982 | 138,732,000 | GCGATGCCTTGTGGGATAC |
| TUP | 138,732,001 | 138,732,024 | TCAGTCCAACCCAGTCACTGCTGC |
| TUR | 138,732,039 | 138,732,061 | CTTGGTCTCTTTGGAGAGGTGTG |
| TDF | 138,743,200 | 138,743,220 | TGCAGGGTAGCTTTGAATGTC |
| TDP | 138,743,227 | 138,743,250 | TGGAGACAGTCCAGACAGCACCTG |
| TDR | 138,743,268 | 138,743,288 | CAGACCCGTCTCCTATTCCAG |
PCR Primers
| Primer | Sequence |
| SU | AGACAACAAGGGGGTGTGG |
| SD | GAGCACCTTTCAGCTCAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14842A-C5 | VGB6 | C57BL/6NTac | |
| 14842A-G2 | VGB6 | C57BL/6NTac | |
| 14842A-G7 | VGB6 | C57BL/6NTac | |
| 14842A-H7 | VGB6 | C57BL/6NTac | |
| 14842A-H10 | VGB6 | C57BL/6NTac | |
| 14842A-C2 | VGB6 | C57BL/6NTac | |
