| Gene | Bpifa2 |
| Chromosome | 2 |
| Deletion Start | 154,009,124 |
| Deletion End | 154,015,372 |
| Deletion Size | 6,249 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 154,009,768 | 154,009,787 | TGTCCCTCAGAACCTGAATC |
| TUP | 154,009,788 | 154,009,813 | TGGATGTGGAGTTGCTTCAGCAAGCC |
| TUR | 154,009,858 | 154,009,877 | AATTGCCAAGGTCCGCTGTG |
| TDF | 154,014,893 | 154,014,914 | GGCTGAGGCTAATCTTGACTTG |
| TDP | 154,014,917 | 154,014,943 | TCCTTCAGCTAATCTACTGGCAGGACA |
| TDR | 154,014,967 | 154,014,988 | TTGTGGCATCCTTCTGTTCTTC |
PCR Primers
| Primer | Sequence |
| SU | AGAAGGTGTGAAGTTTTGCTG |
| SD | AAAGATTAAGTAGAGACCATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14843A-E9 | VGB6 | C57BL/6NTac | |
| 14843A-C3 | VGB6 | C57BL/6NTac | |
| 14843A-D12 | VGB6 | C57BL/6NTac | |
