| Gene | Loxl3 |
| Chromosome | 6 |
| Deletion Start | 83,035,485 |
| Deletion End | 83,050,922 |
| Deletion Size | 15,438 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,036,369 | 83,036,386 | TGTTCCCAGTTGCTCCAC |
| TUP | 83,036,387 | 83,036,416 | TGCTTCAGAGATCAAGTGTCACTTTGGAGA |
| TUR | 83,036,428 | 83,036,451 | TCTCCACTTTCTACTGTTGGTCTC |
| TDF | 83,050,282 | 83,050,305 | TCCCTGACATCTAACACTTCCTTC |
| TDP | 83,050,308 | 83,050,334 | ATGTCTCCAAGAGGTATGAGTGCGCCA |
| TDR | 83,050,341 | 83,050,359 | ACGGTGATGCCCTGCTCTC |
PCR Primers
| Primer | Sequence |
| SU | ATCCGTAGACTCTCCATCTC |
| SD | AACGCGGTCCTTAGGAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14844A-F11 | VGB6 | C57BL/6NTac | |
| 14844A-A11 | VGB6 | C57BL/6NTac | |
| 14844A-E5 | VGB6 | C57BL/6NTac | |
| 14844A-H3 | VGB6 | C57BL/6NTac | |
