| Gene | Cdk5rap1 |
| Chromosome | 2 |
| Deletion Start | 154,370,933 |
| Deletion End | 154,335,462 |
| Deletion Size | 35,472 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 154,370,596 | 154,370,616 | TTTGGCTTCCTTGCTCCACTG |
| TUP | 154,370,569 | 154,370,592 | CCTCTGTGGCCTGATTGCTTCTGC |
| TUR | 154,370,517 | 154,370,540 | CAACCTGCCCACACTATCAACTTC |
| TDF | 154,335,756 | 154,335,773 | CAGGCTGGCTGGATTGTG |
| TDP | 154,335,729 | 154,335,753 | TTCCGTTCTGTCTGGCACCTCTTCC |
| TDR | 154,335,667 | 154,335,688 | GGACAGGACTTGGATGGAGATG |
PCR Primers
| Primer | Sequence |
| SU | AACTGGGTTGTGATTCTGGC |
| SD | AATCAACCAACCAACCAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14847A-B3 | VGB6 | C57BL/6NTac | |
| 14847A-D1 | VGB6 | C57BL/6NTac | |
| 14847A-E12 | VGB6 | C57BL/6NTac | |
