| Gene | Cela2a |
| Chromosome | 4 |
| Deletion Start | 141,825,979 |
| Deletion End | 141,814,937 |
| Deletion Size | 11,043 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,825,146 | 141,825,170 | TGATTGTCCTGGACTCGTTTCTTAG |
| TUP | 141,825,119 | 141,825,145 | AGCAGGCTGGCCTTAAACTCACAGAGA |
| TUR | 141,825,089 | 141,825,109 | CCCAGCACTTCGGAGATAGAG |
| TDF | 141,815,168 | 141,815,186 | GGCCTTCCTGGCCTGATAC |
| TDP | 141,815,143 | 141,815,167 | CCAAGCAGGATTCCGGGCTAAGACC |
| TDR | 141,815,089 | 141,815,107 | TGGAACACTGGGCATTGAG |
PCR Primers
| Primer | Sequence |
| SU | GGTGGTTGTTGCTATTGTCG |
| SD | TCTCCCAATCTCCCATTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14848A-C8 | VGB6 | C57BL/6NTac | |
| 14848A-G10 | VGB6 | C57BL/6NTac | |
| 14848A-E2 | VGB6 | C57BL/6NTac | |
