| Gene | Scrg1 |
| Chromosome | 8 |
| Deletion Start | 57,474,320 |
| Deletion End | 57,477,461 |
| Deletion Size | 3,142 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,474,451 | 57,474,473 | AGCCGACCTGAAGCTGATAGATG |
| TUP | 57,474,478 | 57,474,503 | TGTCCAGCATCATTTCTGGGATGGGA |
| TUR | 57,474,523 | 57,474,545 | GCAGTTCGCTGAAGTTGCAGTAG |
| TDF | 57,476,475 | 57,476,495 | AGGACTGAGTCAGGAGCTGTG |
| TDP | 57,476,496 | 57,476,520 | CAGGCCTTCCCATCAGAGTCCAACA |
| TDR | 57,476,557 | 57,476,580 | CATGTCCTTGCATCATCTAGTGTG |
PCR Primers
| Primer | Sequence |
| SU | TGTAAGTGGTGTCCTGACACTTG |
| SD | ATTGCCTGGTTCTCTGTTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14850A-C5 | VGB6 | C57BL/6NTac | |
| 14850A-D8 | VGB6 | C57BL/6NTac | |
