| Gene | Ccl22 |
| Chromosome | 8 |
| Deletion Start | 94,745,717 |
| Deletion End | 94,749,975 |
| Deletion Size | 4,259 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,745,804 | 94,745,824 | GAGGGTGGAGACCAGAGAATC |
| TUP | 94,745,825 | 94,745,851 | TGGAAAGAGTCCAAGAGTGAGTCTGCC |
| TUR | 94,745,868 | 94,745,891 | GGAAGTGCCAGAGTTAGTCCAAAG |
| TDF | 94,749,554 | 94,749,575 | GCCCTTAGTAGTGTCTGCTTTC |
| TDP | 94,749,577 | 94,749,604 | TGAGCAGATCGGTATGTATCTAGACGGC |
| TDR | 94,749,683 | 94,749,703 | GCTCCTTGTTAGCAAGTCAGC |
PCR Primers
| Primer | Sequence |
| SU | TGTCCTTATCCATGTGCCAG |
| SD | ATGCCCACTGGGACTGATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 150 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14851B-G2 | VGB6 | C57BL/6NTac | |
| 14851B-D3 | VGB6 | C57BL/6NTac | |
