| Gene | Tcn2 |
| Chromosome | 11 |
| Deletion Start | 3,931,453 |
| Deletion End | 3,917,583 |
| Deletion Size | 13,871 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,930,779 | 3,930,799 | CTCCCTGACTGCTCCAGATAG |
| TUP | 3,930,753 | 3,930,778 | TCATCCCAGGGCTATAGTCCTCCAGA |
| TUR | 3,930,675 | 3,930,695 | CGGGTATGACTGTGCCTGAAC |
| TDF | 3,918,035 | 3,918,057 | GTTGCTTCTGGGACACTCATTTG |
| TDP | 3,918,010 | 3,918,034 | TGACACCCTCTGGAACAGTCCCTTT |
| TDR | 3,917,970 | 3,917,988 | GCTTGGCAGGGTCAACATC |
PCR Primers
| Primer | Sequence |
| SU | CTTTAAAGCGTTCGGGACAG |
| SD | AGGCTGCCTTATGACCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 125 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14852A-B4 | VGB6 | C57BL/6NTac | |
| 14852A-H8 | VGB6 | C57BL/6NTac | |
| 14852A-D2 | VGB6 | C57BL/6NTac | |
| 14852A-E12 | VGB6 | C57BL/6NTac | |
| 14852A-D10 | VGB6 | C57BL/6NTac | |
