| Gene | Spock2 |
| Chromosome | 10 |
| Deletion Start | 60,106,471 |
| Deletion End | 60,131,527 |
| Deletion Size | 25,057 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,106,777 | 60,106,797 | CCAGGCTAACTACGCCAAGTG |
| TUP | 60,106,799 | 60,106,822 | AAACAACACCGAGAGTCCCAGCCC |
| TUR | 60,106,863 | 60,106,881 | GAGGCTGCGGGAACTGAAG |
| TDF | 60,131,051 | 60,131,070 | CCCACACTACGGGCTCTTTG |
| TDP | 60,131,071 | 60,131,097 | CCCTAACCCTCAGGTGTCTTCATCCCG |
| TDR | 60,131,131 | 60,131,150 | CCTCTGCTCTGGTCACACTG |
PCR Primers
| Primer | Sequence |
| SU | GGCTTGCAACTCCTTGAGAG |
| SD | GAGGAAGGAAGAAAGGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14859B-B9 | VGB6 | C57BL/6NTac | |
| 14859B-H7 | VGB6 | C57BL/6NTac | |
| 14859B-F9 | VGB6 | C57BL/6NTac | |
| 14859C-B9 | VGB6 | C57BL/6NTac | |
| 14859C-E8 | VGB6 | C57BL/6NTac | |
