| Gene | Otoa |
| Chromosome | 7 |
| Deletion Start | 121,091,547 |
| Deletion End | 121,118,659 |
| Deletion Size | 27,113 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,092,163 | 121,092,186 | TGGTCCCATATCCTGGTAATTGTG |
| TUP | 121,092,192 | 121,092,221 | TTCCACACTGAACACATATGGGCAGATATC |
| TUR | 121,092,244 | 121,092,263 | GCAGCAGAGGCAGCATAGAC |
| TDF | 121,117,944 | 121,117,963 | TAGGAGACGAGGTGGCTTAG |
| TDP | 121,117,964 | 121,117,993 | TGGGATGGGTTTAGATTTAAGTTGCAGCTC |
| TDR | 121,118,021 | 121,118,043 | TGTTCACAGAGACCAGAGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | AAACAACCATCTGCCCTGTC |
| SD | CCCTTAGGATCCATCACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14860A-B5 | VGB6 | C57BL/6NTac | |
| 14860A-C6 | VGB6 | C57BL/6NTac | |
| 14860A-E7 | VGB6 | C57BL/6NTac | |
| 14860A-G2 | VGB6 | C57BL/6NTac | |
| 14860A-G10 | VGB6 | C57BL/6NTac | |
