| Gene | Spink2 |
| Chromosome | 5 |
| Deletion Start | 77,211,371 |
| Deletion End | 77,205,358 |
| Deletion Size | 6,014 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,211,335 | 77,211,355 | TGCTCCTGGTCACAGACTTTG |
| TUP | 77,211,305 | 77,211,330 | TGATGCTCAAGTGGGTCTGGTGTCAA |
| TUR | 77,211,248 | 77,211,270 | GCACGAACCCATCTCCTAACTAC |
| TDF | 77,205,433 | 77,205,451 | CATGCTCGTGGCTGTTCAC |
| TDP | 77,205,406 | 77,205,430 | TGCAGGGAGGACGGTAGCCATATTA |
| TDR | 77,205,364 | 77,205,383 | TTCTGCAAGCTGCCCATCAG |
PCR Primers
| Primer | Sequence |
| SU | AGGGTTGTGTGAAAGGTTGG |
| SD | GGATCCCTGACTGCATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14862A-A6 | VGB6 | C57BL/6NTac | |
| 14862A-H3 | VGB6 | C57BL/6NTac | |
| 14862A-C12 | VGB6 | C57BL/6NTac | |
| 14862A-D10 | VGB6 | C57BL/6NTac | |
| 14862A-G5 | VGB6 | C57BL/6NTac | |
| 14862A-D8 | VGB6 | C57BL/6NTac | |
