| Gene | Prss48 |
| Chromosome | 3 |
| Deletion Start | 86,002,488 |
| Deletion End | 85,993,742 |
| Deletion Size | 8,747 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,002,382 | 86,002,401 | GGGCCTCAGGAAGGAATCAG |
| TUP | 86,002,349 | 86,002,378 | ATAACGTGTGGGCATTACTTGTGAGTGGCA |
| TUR | 86,002,328 | 86,002,346 | TCGCGGGAGTTTCTTGGAC |
| TDF | 85,994,058 | 85,994,077 | GATTCTGGAGGGCCTCTGTC |
| TDP | 85,994,031 | 85,994,056 | TGTCACATTGATGGTGTCTGGAGGCT |
| TDR | 85,993,990 | 85,994,011 | GATCCTTACCGCATTCCAAACC |
PCR Primers
| Primer | Sequence |
| SU | ACTAATGCTCGAATCCTGCC |
| SD | CTGTTGGAGTTCCTTGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14863A-D3 | VGB6 | C57BL/6NTac | |
| 14863A-D10 | VGB6 | C57BL/6NTac | |
| 14863A-E12 | VGB6 | C57BL/6NTac | |
| 14863A-F4 | VGB6 | C57BL/6NTac | |
