| Gene | Oit3 |
| Chromosome | 10 |
| Deletion Start | 59,441,680 |
| Deletion End | 59,423,933 |
| Deletion Size | 17,748 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,441,636 | 59,441,655 | TCTCCACCACAGTCACCTTG |
| TUP | 59,441,613 | 59,441,634 | TGTCACCTGCGGGTAAGTCCCG |
| TUR | 59,441,533 | 59,441,550 | GGCCCTGTCATCTGGTTC |
| TDF | 59,424,629 | 59,424,649 | GACAAGAGGCTGTCCCAAGAG |
| TDP | 59,424,602 | 59,424,624 | TCCCAAGAGGCTGTGGGTGACAA |
| TDR | 59,424,552 | 59,424,574 | TGACCTGAGCTGTGTTTCAATAC |
PCR Primers
| Primer | Sequence |
| SU | TACCGAAGGCTCCGTTACAG |
| SD | GGCCCAGTTACACCACAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14866A-A4 | VGB6 | C57BL/6NTac | |
| 14866A-D10 | VGB6 | C57BL/6NTac | |
| 14866A-F11 | VGB6 | C57BL/6NTac | |
| 14866A-E10 | VGB6 | C57BL/6NTac | |
| 14866A-E7 | VGB6 | C57BL/6NTac | |
| 14866A-G8 | VGB6 | C57BL/6NTac | |
