| Gene | Tex264 |
| Chromosome | 9 |
| Deletion Start | 106,682,122 |
| Deletion End | 106,659,025 |
| Deletion Size | 23,098 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,681,252 | 106,681,272 | TGGGATCTAGTAGGCGTCTGG |
| TUP | 106,681,223 | 106,681,250 | TGTACTAAAGCCTACAGTGAGTCAGGGC |
| TUR | 106,681,193 | 106,681,211 | CCATAGCACGGCAGGAAAC |
| TDF | 106,659,767 | 106,659,786 | GGAGTCCTCTTGGTCCTTTC |
| TDP | 106,659,743 | 106,659,763 | AGCCCTCCTGCCTCTTGGGCA |
| TDR | 106,659,700 | 106,659,720 | TTCAGGCTTGAGAGTGGTTTG |
PCR Primers
| Primer | Sequence |
| SU | CCAGGTATAAACAGGGCTGC |
| SD | GATCCTGCCCATTCACAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14868A-G4 | VGB6 | C57BL/6NTac | |
