| Gene | St6galnac5 |
| Chromosome | 3 |
| Deletion Start | 152,982,014 |
| Deletion End | 152,981,063 |
| Deletion Size | 952 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 152,981,908 | 152,981,929 | GAAGCCTCTCCGGGTATCTATC |
| TUP | 152,981,884 | 152,981,907 | TCTGTGCCAAGAGGTCACCTGTCA |
| TUR | 152,981,850 | 152,981,869 | CCGGATTCCAAGCCAGGTAG |
| TDF | 152,981,491 | 152,981,509 | ACCTGCCCTCCACTGTGAG |
| TDP | 152,981,459 | 152,981,483 | CTGTGAGCAGCGATGCAGGAGCATC |
| TDR | 152,981,427 | 152,981,446 | CAGAGGTGACACGGTCTTGC |
PCR Primers
| Primer | Sequence |
| SU | AAATGGCTACCGTGTTCAGG |
| SD | ACTGAACATCCATCTGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14869A-H8 | VGB6 | C57BL/6NTac | |
| 14869A-G2 | VGB6 | C57BL/6NTac | |
| 14869A-G5 | VGB6 | C57BL/6NTac | |
| 14869A-C5 | VGB6 | C57BL/6NTac | |
