14870   Tbl2     Status: ES cell colonies screened / QC positives

GeneTbl2
Chromosome5
Deletion Start135,149,741
Deletion End135,159,748
Deletion Size10,008
Genome Build38
MGI IDMGI:1351652
NCBI Gene ID27368
BAC ID164K10
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,149,926135,149,944ACGAGGAGCGTGGAGAGTC
TUP135,149,946135,149,970CGGACCTCAGTTTCCCTGGTTGTGA
TUR135,149,977135,149,998ACCCGAAACCTACACGGGAAAG
TDF135,159,036135,159,054AGCTGGGCTGAGCAGTGAG
TDP135,159,062135,159,085TACTTCTGCCTGTCTGGGCTGCAG
TDR135,159,110135,159,128GGTTACATCCTGCCGAGAC

PCR Primers

PrimerSequence
SUGCTCCGCCCTTATAGAGTTC
SDGGTCCAGATGGAGGCTCAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR73 bp++-
TDFTDR93 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
14870A-A6VGB6C57BL/6NTac
14870A-H5VGB6C57BL/6NTac
14870A-E3VGB6C57BL/6NTac
14870A-H10VGB6C57BL/6NTac
14870A-C6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image