| Gene | Igfbpl1 |
| Chromosome | 4 |
| Deletion Start | 45,826,790 |
| Deletion End | 45,813,380 |
| Deletion Size | 13,411 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,826,206 | 45,826,225 | AGCAGTGTTTCGCGTCTTTC |
| TUP | 45,826,181 | 45,826,204 | ACAGCCTGCTTGCTACCTTACCCA |
| TUR | 45,826,149 | 45,826,171 | CTGTTTGTTCTAGGGCTGAAGTC |
| TDF | 45,813,937 | 45,813,956 | TCAGCCCTTGTCGCCTTTAG |
| TDP | 45,813,912 | 45,813,936 | TGAAAGGACTGGGCAGCTCACAGTA |
| TDR | 45,813,873 | 45,813,891 | CCTGCTGCTCCTGTGTCTG |
PCR Primers
| Primer | Sequence |
| SU | TCGATCTGGGAAAGAAATCC |
| SD | GGTGGGACAAATACACAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14871A-D10 | VGB6 | C57BL/6NTac | |
| 14871A-G8 | VGB6 | C57BL/6NTac | |
| 14871A-F10 | VGB6 | C57BL/6NTac | |
| 14871A-E7 | VGB6 | C57BL/6NTac | |
