| Gene | Myg1 |
| Chromosome | 15 |
| Deletion Start | 102,331,744 |
| Deletion End | 102,337,835 |
| Deletion Size | 6,092 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,331,752 | 102,331,776 | TTTCCTGCGTGGTATCTTAACATTG |
| TUP | 102,331,778 | 102,331,800 | CGCTCCGGTCAGTTCTCCAAGCC |
| TUR | 102,331,844 | 102,331,861 | TGAGGTTGTTGCGAGGTC |
| TDF | 102,337,026 | 102,337,049 | GGTTGATCTTCCAATCCCTGTGAG |
| TDP | 102,337,055 | 102,337,084 | ATCTGGGCCAGTGTTCAACATGTCTTGTCA |
| TDR | 102,337,106 | 102,337,126 | AGGTCCATTGCTCGCCTGAAC |
PCR Primers
| Primer | Sequence |
| SU | GCACTGCAGAAATGAAGCAG |
| SD | TCTTCCAGGACAATCATCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14872A-A5 | VGB6 | C57BL/6NTac | |
| 14872A-B3 | VGB6 | C57BL/6NTac | |
| 14872A-F3 | VGB6 | C57BL/6NTac | |
| 14872A-E9 | VGB6 | C57BL/6NTac | |
