| Gene | Kdelc1 |
| Chromosome | 1 |
| Deletion Start | 44,118,496 |
| Deletion End | 44,107,003 |
| Deletion Size | 11,494 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,118,350 | 44,118,368 | CCTTCCTGCCCGCTATTTC |
| TUP | 44,118,326 | 44,118,348 | ACATTCGGGCGGTGGATACCTCA |
| TUR | 44,118,298 | 44,118,322 | GGAGTCATTTCTCCACTTACTGTTC |
| TDF | 44,107,820 | 44,107,844 | CCCAAATTCGAGAGGGTATGAAGAG |
| TDP | 44,107,792 | 44,107,817 | TAGAGCCACAATCCGAGGATGACCTC |
| TDR | 44,107,739 | 44,107,757 | GGGAGGCAGAAAGTGAGTC |
PCR Primers
| Primer | Sequence |
| SU | CCTTGGCTAGAACTTGCGAC |
| SD | TTTGGAGGCCTGTTTGAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14874A-H1 | VGB6 | C57BL/6NTac | |
| 14874D-E4 | VGB6 | C57BL/6NTac | |
| 14874D-E5 | VGB6 | C57BL/6NTac | |
